Skip to main content

Mir1-CP in combination with Bt toxin provide ultimate protection

A cocktail of insecticides containing a plant protein and a common insecticide may be more lethal to crop pests than either ingredient used alone, according to biologists. The one-two punch also inhibits the insects' growth rate and reduces their chance of developing resistance. "We found a synergistic effect, where the two insecticides together decreased the growth rate of caterpillars more than either one did alone," said Dawn Luthe, professor of plant stress biology at Penn State. "The insect mortality rate was also much greater than the sum of mortality rates when only one insecticide was used." One of those insecticides, Bt, is commonly used around the world. When insects feed on plants genetically modified to produce Bt, the toxin binds to chemical receptors lining the insects' midgut. This disrupts the receptor's function, eventually killing the insects. But researchers say some insects always survive the ordeal and over time subsequent populations could develop resistance to the toxin. Luthe and her colleagues Srinidhi Mohan and Peter W. K. Ma, post-doctoral student and professor at Mississippi State University, and W. Paul Williams, research geneticist at the U.S Department of Agriculture studied a unique plant-based insecticide known as Mir1-CP.

Their goal is to see if Mir1-CP, when used in tandem with other biological pesticides, such as the Bt toxin, can prevent pests from developing resistance and make the toxin more effective. "This protein, which we developed from certain strains of corn from Antigua, breaks down other proteins and peptides in the insects' gut," said Luthe, whose findings appear in the current issue of PLoS One. Unlike Bt, Mir1-CP breaks down proteins in a protective membrane covering the midgut. This membrane acts as a barrier that protects the caterpillar from toxins in the diet, and cycles nutrients to the midgut. "It is the caterpillar's first line of defense against toxins and chemicals in its diet," said Luthe. The researchers fed insects a sub-lethal dose of the two insecticides to test the effectiveness of both insecticides on the pests.

They found that when used alone, a concentration of Bt at five parts per billion killed four percent of all corn earworms and
five per cent of tobacco budworms. Mir1-CP, when used at a concentration of 60 parts per billion, killed eight percent of the corn earworms and three percent of the tobacco budworms. But when researchers added the two insecticides together, the mixture killed 61 per cent of corn earworms and 57 percent of tobacco budworms, which is more than 10 times better than either by itself. Researchers saw similar results against the fall armyworm and the southwest corn borer, when the insecticides were used at slightly different strengths. In addition to a high mortality rate among the insects, the study indicates a significant decrease in the growth rate of the survivors. "We think that Mir1-CP is making holes in the membrane, which in turn is making it easier for the Bt toxins to reach the insects' midgut," explained Luthe, whose work is funded by the National Science Foundation.

The Penn State researcher says the findings have important implications for agriculture because each year insects cause major losses to farmers. Nearly 20 per cent of major crops worldwide are lost to insects. Genetically modified crops that produce the Bt toxin have managed to check the insects to some extent but Luthe says insects may be winning the fight. "Researchers in the Mississippi delta have found resistance to Bt among some insect populations in the region," Luthe noted. "There is a chance that some time in the future Bt will not be as effective against pests as it is now." The Penn State researcher suggests strains of corn that naturally produce Mir1-Cp could be cross-bred with other strains of corn that produce Bt to develop new varieties that are more effective against pests. "If you have two mechanisms of attack, it will take much longer for an insect population to develop resistance to both modes of attack, and help slow the development of resistance," Luthe added. Credit: Sciencedaily and TAMU.

Comments

Popular posts from this blog

Charging Implanted Heart Pumps Wirelessly

Mechanical pumps to give failing hearts a boost were originally developed as temporary measures for patients awaiting a heart transplant. But as the technology has improved, these ventricular assist devices commonly operate in patients for years, including in former vice-president Dick Cheney, whose implant this month celebrates its one-year anniversary. Prolonged use, however, has its own problems. The power cord that protrudes through the patient's belly is cumbersome and prone to infection over time. Infections occur in close to 40 percent of patients, are the leading cause of rehospitalization, and can be fatal. Researchers at the University of Washington and the University of Pittsburgh Medical Center have tested a wireless power system for ventricular assist devices. They recently presented the work in Washington, D.C. at the American Society for Artificial Internal Organs annual meeting, where it received the Willem Kolff/Donald B. Olsen Award for most promising research in

Autism and Eye Contact: Genes very much are involved

We have now a lot of evidence on genetic components in many disorders including neurological in both adults and kids. Autism is one such problem that has many genes involved. Research is still in full swing to find more genes and related pathways. However, one can find autistic features more phenotypically before genotyping. Eye contact is one of them. Studies have shown that autistic kids make less eye contact. This has been shown to have genetic component now. New research has uncovered compelling evidence that genetics plays a major role in how children look at the world and whether they have a preference for gazing at people's eyes and faces or at objects. The discovery by researchers at Washington University School of Medicine in St. Louis and Emory University School of Medicine in Atlanta adds new detail to understanding the causes of autism spectrum disorder. The results show that the moment-to-moment movements of children's eyes as they seek visual information about the

How much people depend on weather reports

Meteorologists on television, radio, online, and in newspapers supply weather reports to the average person over 100 times a month. Surveys demonstrated that the 300 billion forecasts accessed generate a value of $285 per household every year, or $32 billion for the entire United States. Odds are you have already watched one weather forecast today and will probably check out a few more. Accurate, timely forecasts are vital to everyday life, but just how critical may surprise you. Whether at work or play, you probably watch the weather quite closely. Most of us are at the weather person's mercy to know what to wear, what to expect, to prepare for the worst. New research shows the average United States household checks out a weather report more than three times a day. "It impacts pretty much every part of every activity we are involved with for the most part," Jeff Lazo, the director of the Societal Impacts Program at the National Center for Atmospheric Research (NCAR) in B